The Effects of Resistance Training and Berberine Chloride Supplementation on Oxidative Stress Markers in the Cerebellum Tissue of Diazinon-Poisoned Rats

Background: Diazinon is an insecticide from the organophosphorus group, which can increase the oxidative stress in the body. It has been established that training and antioxidant supplementation can improve oxidative stress system by decreasing malondi-aldehyde (MDA); meanwhile, increasing the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GP X ), and catalase (CAT). Objectives: The aim of this study was to investigate the effect of resistance training (RT) and berberine chloride (BC) supplementation on oxidative stress indices in the cerebellum tissue of diazinon-poisoned rats. Methods: Fifty-six Wistar rats were divided into seven groups (n = 8), including resistance training (RT) (each session 2 sets of 6 repetitions, each one required 8 to 12 active movements with intensity of 10% - 50% of body weight, 3 sessions per week), BC2 (BC: 2 mg/kg), BC15 (BC: 15 mg/kg), RT + BC2, RT + BC15, diazinon, and control. MDA, SOD, and CAT were measured in the cerebellum tissue. Results: RTandBCsignificantlyreducedMDAproteinlevels(P< 0.01),whileincreasedSODproteinlevels(P< 0.01)andCATprotein levels(P< 0.01)inthecerebellumtissueof diazinon-poisonedrats; RTwithBChadinteractiveeffectsondecreasingMDA(P< 0.01), while increasing SOD (P < 0.01) and CAT protein levels (P < 0.01). Also, the consumption of 15 mg/kg BC rather than 2 mg/kg BC significantly increased SOD protein levels (P = 0.02). Conclusions: It seems that RT combination with BC has Interactive effects on the improvement of oxidative stress markers in the cerebellum tissue of diazinon-poisoned rats. macro-moleculesorcellmicro-moleculescausingcellandgenetic damages (6). Some researchers suggest that an increase in lipid peroxidation and the production of free radicals derived from the metabolism of organophosphorus pesticides are the main mechanisms of destruction in various cellsandtissuesof thebody(7); consequently,thebraintis-sue appears to be one of the organs, which is affected by these toxins. In this regard, studies have shown that some organophosphorus products, by increasing the productionof freeradicals,canaltertheactivityof antioxidanten-zymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and increase the malondialde-hyde (MDA) in various tissues (8). There are several strate-gies to improve the antioxidant enzymes, including sports activities and antioxidant supplementation.