Explain a) Tet-On and b) Tet-Off system. Describe the proteins involved, the DNA sequence of the regulatory elements, and the mechanisms. - tlooto, The Most Powerful AcademicGPT

Tet-Off: tTA (TetR + VP16) binds TRE (multi tetO upstream of minimal promoter) to activate transcription; doxycycline binding disables tTA, turning OFF. Tet-On: rtTA (mutant TetR + VP16) binds TRE onl - tlooto, AI-Powered Assistant for Academic and Research