You have requested to find and obtain the article titled Induction of Chronic Subclinical Systemic Inflammation in Sprague-Dawley Rats Stimulated by Intermittent Bolus Injection of Lipopolysaccharide. Based on the information and context provided, I am unable to directly deliver the full text of this article. However, I can guide you efficiently in your search and provide relevant background about the topic as reflected by published research in Sprague-Dawley rats using lipopolysaccharide (LPS) to induce subclinical or systemic inflammation.
Sprague-Dawley rats are one of the most widely used outbred laboratory rat strains for biomedical research, including studies of inflammation, due to their well-characterized genetics and physiology[1]. LPS, a component of the outer membrane of Gram-negative bacteria, is commonly utilized to experimentally induce systemic inflammation and immune activation in rodents. Previous studies have established that repeated or intermittent administration of LPS in Sprague-Dawley rats can produce both acute and chronic inflammatory responses, manifesting in altered immune organ indices, changes in blood parameters, and suppressed growth, which are indicative of sustained immune activation and subclinical or chronic inflammatory states[2][3]. Research has also shown that such LPS-induced systemic inflammation can result in organ-specific physiological and molecular changes, including effects on vascular endothelium, brain, and other tissues, further supporting the use of this model for studying chronic inflammation and its systemic consequences in rats[3][4][5]. If you are seeking the full text of the specific article, it may be accessed through academic databases or by request from your institution's library services.
Reference selection rationale:References were selected according to their direct relevance to the induction of systemic or subclinical inflammation in Sprague-Dawley rats using LPS, as described in your paragraph. Article [1] provides background on the wide usage and characterization of Sprague-Dawley rats in research, establishing the appropriateness of the model. Article [2] specifically investigates the effects of repeated LPS injections in Sprague-Dawley rats, observing clear markers of chronic immune activation and systemic inflammation. Article [3] demonstrates the protocol and consequences of chronic LPS administration, detailing both endothelial dysfunction and systemic inflammatory responses, which mirror the chronic subclinical phenotype discussed. Articles [4] and [5] elaborate on the long-term or molecular changes following LPS-induced systemic inflammation in rats, substantiating its application for modeling persistent immune activation. These references were drawn from your provided list due to their methodological and thematic alignment with LPS-induced systemic or chronic inflammation models in Sprague-Dawley rats, and their findings directly support the statements in your paragraph.
